Research published by Dr. James Piret and former CHBE PhD Student Mario Jardon recognized as Top Cited Research by the journal Biotechnology & Bioengineering

The article “Inhibition of glutamine-dependent autophagy increases t-PA production in CHO cell fed-batch processes”, published in the journal Biotechnology & Bioengineering (Vol. 109, May 2012, pages 1228–1238) has been recognized as Top Cited Research by the journal. The article presents the results of a multi-disciplinary study led by Dr. Piret’s group in collaboration with the groups of Dr. Michael Butler (Microbiology, University of Manitoba), Dr. Hélène Côté (Pathology, University of British Columbia) and Dr. Sharon Gorski (Genome Sciences Centre, BC Cancer Agency, and Molecular Biology & Biochemistry, Simon Fraser University).

Upon publication, this manuscript was showcased in the Spotlight section of the journal, with the following mention:
Autophagy is a cell survival mechanism resulting in lysosomal digestion and reuse of cytoplasmic components in response to stresses such as nutrient limitation. It is ubiquitous in eukaryotes and has been implicated in many diseases including cancer, inflammatory and neurodegenerative disorders. The analysis of CHO cell physiology in fed-batch processes by Jardon and coworkers indicated that autophagy can also strongly influence the production of therapeutic recombinant proteins. CHO cell autophagy induced by glutamine inhibition was successfully inhibited by 3-methyl adenine to increase the tissue plasminogen activator yield in fed-batch cultures by 2.8-fold, up to 500 mg/L. This treatment and remarkable increase in production did not diminish the CHO cell recombinant protein glycosylation and instead the fucosylation, galactosylation, and sialylation were increased. This work should stimulate future studies aiming to increase the yield and quality of recombinant protein by modulating the cellular process of autophagy. (Biotechnology and Bioengineering, Vol. 109, No. 5, May, 2012, Spotlight).

Biotechnology & Bioengineering is a leading journal publishing Articles, Communications to the Editor, Reviews, Mini-Reviews, and Perspectives that contribute to the advancement of biotechnology, including cell culture engineering, vaccine technology, renewable resource engineering, and more.

The full notice can be accessed at:

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