Dr. Kryworuchko is an Immunologist who joined UBC and the BCCDC at the end of 2017. His research aims to better understand host-pathogen interactions at the cellular and molecular levels with the ultimate goal of developing new and more effective infectious disease therapeutics and vaccines. Recent efforts have been focussing on the cellular autophagy or “self-eating” pathway. Autophagy, as its name suggests, allows the cell to engulf and degrade portions of its cytoplasm for use as an energy source, and to remove superfluous or defective organelles and proteins. However, the autophagy machinery of the cell is also critical for the elimination of certain intracellular pathogens such as Mycobacterium tuberculosis and HIV. Autophagy signals the presence of pathogen to the immune system, and mobilizes innate and adaptive immune cells including monocytes/macrophages, helper and killer T cells, as well as antibody-producing B lymphocytes. Recent research focusses on the design and evaluation of autophagy-based therapeutics and vaccines against pathogens of major public health importance including Chlamydia, as well as Salmonella and other organisms resistant to currently available antimicrobial treatments.
Immunoengineering (BMEG 591I – Topics in Biomedical Engineering) – This graduate course delves into critical analyses of selected, leading-edge platforms being developed to harness the immune system for the prevention and/or control of diseases such as infection and cancer. Areas for in-depth analysis include nanoscale vaccines and mucosal delivery systems, ‘-omics’-based target discovery, engineered immune cells and molecules, and recent advances in upstream or downstream immunotherapeutic production processes. Topics are selected in class, based on student interest.
Please contact Dr. Kryworuchko by Email for undergraduate and graduate student training opportunities in these exciting areas of research & development.
Selected Papers & Presentations
1. Braasch, K, Kryworuchko, M.*, and Piret, J. Autophagy enhances exogenous monoclonal antibody production in a mammalian cell culture system. Paper in preparation.
2. Alhetheel, A., Bridge, R., Elsageyer, M., Kumar, A., and Kryworuchko, M.* Autophagy is implicated in a caspase-independent form of spontaneous and Interferon-induced programmed cell death that is attenuated by Interleukin-10 in human monocytes. Paper submitted.
3. Benoit, A., Saxena, M., Saad, A., Kumar, A., and Kryworuchko, M.* Selective role of Growth Factor Independent -1 (GFI1) in IL-4-dependent repression of IL-7R expression and proliferation of primary human CD8 T lymphocytes. Paper submitted.
4. Autophagy: a little bit of cellular “self-eating” to help fight infection. 2017. BC Centre for Disease Control Research Week, Vancouver, BC. Seminar.
5. Braash, K., Kryworuchko, M.,* Piret, J. 2017. Autophagy enhances exogenous monoclonal antibody production in a mammalian cell culture system. Vancouver Autophagy Symposium, Vancouver, BC. Poster.
6. Therapeutic targeting of the autophagy pathway as anti-microbial agent and vaccine adjuvant. 2015. Centre for Drug Research and Development, Biologics Group, Vancouver, BC. Seminar.
7. Pujhari, S., Kryworuchko, M., and Zakhartchouk, A. 2014. Role of phosphatidylinositol-3-kinase (PI3K) and the mammalian target of rapamycin (mTOR) signaling pathways in porcine reproductive and respiratory syndrome virus (PRRSV) replication. Virus Research. 194:138-44.
8. Life or death decisions of autophagy. 2014. Canadian Society for Immunology Conference, Quebec City, Quebec, Symposium Seminar.
9. Alhetheel, A., Aly, M., and Kryworuchko, M. 2013. Immune responses and cell signaling during chronic HIV infection. In “Current Perspectives in HIV Infection” (InTech)
10. Role of autophagy in monocyte programmed cell death: implications for chronic HIV infection. 2013. University of Peruana Cayetano Heredia, Lima, Peru, Seminar.
11. Life or death decision of autophagy across our kingdoms. 2013. Brock University, Department of Biological Sciences, St. Catharines, ON, Seminar.
12. Saxena, M., Busca, A., Pandey, S., Kryworuchko, M., and Kumar, A. 2011. CpG protects human monocytic cells against HIV-vpr-induced apoptosis by c-IAP-2 through the calcium-activated JNK pathway in a TLR-9 independent manner. J. Immunol. 187(11):5865-5878.
13. Benoit, A., Abdkader, K., Sirskyj, D., Alhetheel, A., Sant, N., Diaz-Mitoma, F., Kumar A., and Kryworuchko, M.* 2009. Inverse association of repressor Gfi-1 with CD8 T cell IL-7R expression and limited STAT signaling in response to IL-7 among c cytokines in HIV patients. AIDS. 23(11):1341-7.
14. Busca, A., Saxena, M., Kryworuchko, M., and Kumar, A. 2009. Anti-apoptotic genes in the survival of monocytic cells during infection. Current Genomics. 10(5): 306-317.
15. Alhetheel, A., Yakubtsov, Y., Abdkader, K., Sant, N., Diaz-Mitoma, F., Kumar, A., and Kryworuchko, M.* 2008. Amplification of the STAT1 signaling pathway and its association with apoptosis in monocytes from HIV-infected patients. AIDS. 22: 1137-1144.
16. Sirskyj, D., Kumar, A., Theze, J., and Kryworuchko, M.* 2008. Disruption of the γc cytokine network in T cells during HIV infection. Cytokine. 43: 1–14.
17. HIV-induced monocytic cell death & blood-borne pathogen diagnostics in a NANO-nutshell. 2008. Canadian Blood Services. Ottawa, ON, Seminar.
18. Azizi, A., Ghorbani, M., Kryworuchko, M., Aucoin, S., Diaz-Mitoma F. 2005. Potency of cell-mediated immune responses to different combined HIV immunogens in a humanized murine model. Human Vaccines. 1(4): e24-e30.
19. Kryworuchko, M., Pasquier, V., Keller, H., David, D., Gilquin, J., Viard, J.P., Ranoux, M.T., Joussemet, M., Delfraissy J.F., Theze, J. 2004. Defective IL-2-dependent STAT5 signaling in the CD8 T lymphocytes from HIV+ patients: restoration by HAART. AIDS. 18: 421-426.
20. Kryworuchko, M., Creery, D., Kumar, A., 2004. The role of Interleukin-10 in infectious diseases. Interleukin-10, F. Marincola, ed., Landes Bioscience, pubs. pp. 107-124.
• For a list of publications, see Google Scholar